This Vitis mapping population consisted of F1 hybrids derived from a cross between female parent ‘Chambourcin’ with a pollen donor ‘Cabernet Sauvignon’. These hybrids were planted at the Missouri State Fruit Experiment Station (MSFES), Mountain Grove, MO 65711 (latitude 37°09'14.8"N; longitude 92°14'46.8" W). An initial planting in 2014 contained 100 progeny, followed by 26 progeny in 2015 and 180 progeny in 2016. The data set includes microsatellite (SSR) and core genome rhAmpSeq haplotype marker data that was used to develop genetic map and phenotype data generated from Blackbird microscopy robot analysis of grapevine powdery mildew (Erysiphe necator) severity.